Real-Time PCR Method for Detection of Salmonella spp. in Environmental Samples
نویسندگان
چکیده
منابع مشابه
Detection and enumeration of Cryptosporidium oocysts in environmental water samples by Real-time PCR assay
Introduction: The protozoan parasite, Cryptosporidium Spp., widely spreads in both raw and drinking waters. It is the causative agents of waterborne diarrhea and gastroenteritis in the world. In the present study, a molecular assay was used for the detection and quantification of Cryptosporidium oocysts in environmental water samples. Materials and methods: Thirty surface water samples wer...
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introduction: the protozoan parasite, cryptosporidium spp., widely spreads in both raw and drinking waters. it is the causative agents of waterborne diarrhea and gastroenteritis in the world. in the present study, a molecular assay was used for the detection and quantification of cryptosporidium oocysts in environmental water samples. materials and methods: thirty surface water samples were col...
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A robust 5' nuclease (TaqMan) real-time PCR was developed and validated in-house for the specific detection of Salmonella in food. The assay used specifically designed primers and a probe target within the ttrRSBCA locus, which is located near the Salmonella pathogenicity island 2 at centisome 30.5. It is required for tetrathionate respiration in Salmonella. The assay correctly identified all 1...
متن کاملDetection and differentiation of Cryptosporidium spp. in human clinical samples by use of real-time PCR.
Real-time PCR has the potential to streamline detection and identification of Cryptosporidium spp. in human clinical samples. In the present article, we report the first such assay to allow not only detection and differentiation of the most common human pathogens, Cryptosporidium hominis and Cryptosporidium parvum, but also simultaneous amplification of a region of the small subunit (SSU) rRNA ...
متن کاملSalmonella detection in poultry samples. Comparison of two commercial real-time PCR systems with culture methods for the detection of Salmonella spp. in environmental and fecal samples of poultry.
STUDY The efficiency of two commercial PCR methods based on real-time technology, the foodproof® Salmonella detection system and the BAX® PCR Assay Salmonella system was compared to standardized culture methods (EN ISO 6579:2002 - Annex D) for the detection of Salmonella spp. in poultry samples. MATERIAL AND METHODS Four sample matrices (feed, dust, boot swabs, feces) obtained directly from p...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 2017
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.00644-17